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Invitrogen
PDK2 Polyclonal Antibody
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FIGURE: 1 / 2
PDK2 Antibody (PA5-75472) in WB
Western blot analysis of PDK2 in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: L02 whole cell lysate (40 µg), Lane 3: the brain tissue lysate of mouse (40 µg), Lane 4: the brain tissue lysate of rat (40 µg), Lane 5: the liver tissue lysate of mouse (40 µg), Lane 6: the liver tissue lysate of rat (40 µg). Samples were incubated with PDK2 polyclonal antibody (Product # PA5-75472) at a dilution of 1:500.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-75472
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 260-322 of Human PDK2.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; PDH kinase 2; PDK P45; pyruvate dehydrogenase; pyruvate dehydrogenase 2; pyruvate dehydrogenase kinase 2 subunit p45 (PDK2); Pyruvate dehydrogenase kinase isoform 2; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase kinase, isozyme 2
Gene Aliases: PDHK2; PDK2
UniProt ID: (Rat) Q64536, (Mouse) Q9JK42
Entrez Gene ID: (Rat) 81530, (Mouse) 18604
protein serine/threonine kinase activity
pyruvate dehydrogenase (acetyl-transferring) kinase activity
ATP binding
protein complex binding
protein homodimerization activity
protein heterodimerization activity
nucleotide binding
protein binding
kinase activity
transferase activity
non-receptor serine/threonine protein kinase
glucose metabolic process
regulation of gluconeogenesis
protein phosphorylation
regulation of pH
insulin receptor signaling pathway
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of cellular ketone metabolic process
regulation of glucose metabolic process
peptidyl-serine phosphorylation
cellular response to nutrient
cellular response to reactive oxygen species
glucose homeostasis
intrinsic apoptotic signaling pathway by p53 class mediator
carbohydrate metabolic process
phosphorylation
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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