Invitrogen

COPB2 Polyclonal Antibody

Name: COPB2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-77106
Availability: InStock

高级验证

1 篇参考文献

查看其他8个COPB2抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)
高级验证 (1)

COPB2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

COPB2 Antibody (PA5-77106) in ICC/IF

Immunofluorescence analysis of COPB2 in U20S cells. Samples were incubated with COPB2 polyclonal antibody (Product # PA5-77106) followed by DAPI (nuclear staining).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

COPB2 Antibody — Figure 1 The expression levels of COPB2 in human adenocarcinoma cell lines and normal bronchial epithelial cells and the effects of knockdown COPB2 on cell proliferation, apoptosis, and migration in NCI-H1975 human adenocarcinoma cells. ( A-C ) Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) show the expression level of COPB2 in BEAS-2B normal human bronchial epithelial cells and human lung adenocarcinoma cells, NCI-H1299, A549, SK-MES-1, NCI-H1688, and NCI-H1975. * P <0.05, *** P <0.001 vs. BEAS-2B. ( D-F ) Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) show the relative expression level of COPB2 in NCI-H1975 cells. ( G ) The apoptosis rate of NCI-H1975 cells was measured by flow cytometry. The percentage of apoptotic cells, determined by propidium iodide (PI) + /Annexin V + , is shown in the right chart. ( H ) Photomicrograph of the transwell assay for NCI-H1975 cells. Magnification x200. The migration rate of NCI-H1975 cells is shown in the right chart. ( I ) Photomicrograph of the transwell assay for NCI-H1975 cells. Magnification x200. The invasion rate of NCI-H1975 cells is shown in the right chart. ( J ) Cell proliferation of NCI-H1975 cells detected by the Cell Counting Kit-8 (CCK-8) assay. *** P <0.001 vs. NC. Control - fluorescence-labeled transfection control siRNA; NC - scrambled negative control siRNA; siCOPB2 - siRNA for knockdown of COPB2. {KD}

Immunogen image

Immunogen Image COPB2 Polyclonal Antibody

产品信息

PA5-77106

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length Human COPB2.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2720833

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。