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Invitrogen
alpha Actinin 3 Polyclonal Antibody
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alpha Actinin 3 Antibody (PA5-78720) in IHC (P)
Immunohistochemistry analysis of alpha Actinin 3 on paraffin-embedded mouse skeletal muscle tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with alpha Actinin 3 polyclonal antibody (Product# PA5-78720) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-78720
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3 (574-617aa EADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINT K).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HT1080 whole cell, rat skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse skeletal muscle tissue. IHC: mouse skeletal muscle tissue, rat skeletal muscle tissue, human lung cancer tissue. Flow: WISH cell.
靶标信息
Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Actinin alpha3; Actinin-alpha3; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-3; alpha-Actinin3; alphaActinin-3; F-actin cross-linking protein
基因别名: ACTN3
UniProt ID: (Mouse) O88990
Entrez Gene ID: (Mouse) 11474, (Rat) 171009
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