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Invitrogen
AHA1 Polyclonal Antibody
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图: 1 / 1
AHA1 Antibody (PA5-78743) in WB
Western blot analysis of AHA1 in Lane 1: rat brain tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: rat testis tissue lysate, Lane 4: human A375 cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with AHA1 polyclonal antibody (Product # PA5-78743) ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-78743
产品规格
种属反应
Human,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human AHA1 recombinant protein (Position: N21-F338).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human PC-3 whole cell, human A549 whole cell, monkey COS-7 whole cell.
靶标信息
Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activator of 90 kDa heat shock protein ATPase homolog 1; Aha 1; AHA1; AHSA 1; p38; SHSA1
基因别名: AHSA1; C14orf3; HSPC322
Entrez Gene ID: (Human) 10598
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