Invitrogen

APOE Polyclonal Antibody

查看其他34个APOE抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (6)

APOE Antibody in Immunocytochemistry (ICC/IF)

APOE Antibody in Immunocytochemistry (ICC/IF)

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APOE Antibody (PA5-78803) in ICC/IF

Immunocytochemistry analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (Product # PA5-78803) . Apolipoprotein E/APOE was detected in a section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-Apolipoprotein E/APOE antibody (Product # PA5-78803) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated ... View More

APOE Antibody in Immunocytochemistry (ICC/IF)

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOE Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

APOE Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (Product # PA5-78803) . Apolipoprotein E/APOE was detected in a section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-Apolipoprotein E/APOE antibody (Product # PA5-78803) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of APOE in paraffin-embedded section of mouse liver tissue using APOE Polyclonal Antibody (Product # PA5-78803). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 5 µg/mL dilution overnight at 4°C. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of APOE in paraffin-embedded section of rat liver tissue using APOE Polyclonal Antibody (Product # PA5-78803). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 5 µg/mL dilution overnight at 4°C. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of APOE on paraffin-embedded mouse liver tissue. Sample was incubated with APOE polyclonal antibody (Product# PA5-78803) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of APOE on paraffin-embedded rat liver tissue. Sample was incubated with APOE polyclonal antibody (Product# PA5-78803) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

APOE Antibody in Western Blot (WB) — Western blot analysis of APOE in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Sample was incubated with APOE polyclonal antibody (Product # PA5-78803) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

Immunogen image

APOE Polyclonal Antibody

Immunogen Image APOE Polyclonal Antibody

产品信息

PA5-78803

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

免疫细胞化学 (ICC/IF)

5 µg/mL

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: mouse spleen tissue, mouse kidney tissue. IHC: mouse liver tissue, rat liver tissue. ICC/IF: HEPA1-6 cell.

靶标信息

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571

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基因别名: AI255918; Apo-E; Apoe; APOEA

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UniProt ID: (Mouse) P08226, (Rat) P02650

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Entrez Gene ID: (Mouse) 11816, (Rat) 25728

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beta-amyloid binding receptor binding lipid transporter activity phospholipid binding heparin binding lipid binding cholesterol binding antioxidant activity cholesterol transporter activity identical protein binding protein homodimerization activity hydroxyapatite binding metal chelating activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding apolipoprotein

negative regulation of endothelial cell proliferation response to dietary excess lipid metabolic process triglyceride metabolic process cholesterol biosynthetic process cholesterol catabolic process lipid transport cellular calcium ion homeostasis receptor-mediated endocytosis response to oxidative stress G-protein coupled receptor signaling pathway nitric oxide mediated signal transduction aging protein localization cholesterol metabolic process regulation of gene expression negative regulation of platelet activation positive regulation of cholesterol esterification positive regulation of cholesterol efflux peripheral nervous system axon regeneration long-chain fatty acid transport protein import triglyceride catabolic process cGMP-mediated signaling negative regulation of blood coagulation positive regulation of cGMP biosynthetic process neuron projection regeneration axon regeneration regulation of cholesterol transport regulation of Cdc42 protein signal transduction response to retinoic acid positive regulation of low-density lipoprotein particle receptor catabolic process response to insulin cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process vasodilation cholesterol homeostasis positive regulation of catalytic activity negative regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration reverse cholesterol transport response to ethanol negative regulation of cholesterol biosynthetic process positive regulation of axon extension positive regulation of lipid biosynthetic process oligodendrocyte differentiation artery morphogenesis negative regulation of inflammatory response positive regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis negative regulation of lipid biosynthetic process maintenance of location in cell lipid homeostasis fatty acid homeostasis positive regulation of dendritic spine development negative regulation of dendritic spine development cellular response to interleukin-1 cellular response to growth factor stimulus cellular response to cholesterol cardiovascular system development negative regulation of cholesterol efflux AMPA glutamate receptor clustering NMDA glutamate receptor clustering regulation of beta-amyloid clearance regulation of neuron death negative regulation of neuron death positive regulation of neuron death negative regulation of postsynaptic membrane organization positive regulation of postsynaptic membrane organization negative regulation of presynaptic membrane organization positive regulation of presynaptic membrane organization positive regulation of beta-amyloid formation negative regulation of beta-amyloid formation regulation of tau-protein kinase activity negative regulation of dendritic spine maintenance positive regulation of dendritic spine maintenance positive regulation of phospholipid efflux positive regulation of neurofibrillary tangle assembly negative regulation of phospholipid efflux negative regulation of lipid transport across blood brain barrier positive regulation of lipid transport across blood brain barrier