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Invitrogen
Aquaporin 8 Polyclonal Antibody
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FIGURE: 1 / 2
Aquaporin 8 Antibody (PA5-78817) in WB
Western blot analysis of AQP8 in, Lane 1: human PC-3 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with Aquaporin 8 Polyclonal Antibody (Product # PA5-78817) at 0.5 μ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78817
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Aquaporin 8 (1-19aa MSGEIAMCEPEFGNDKARE).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human PC-3 whole cell.
Target Information
Aquaporin-8 is a protein that in humans is encoded by the AQP8 gene. Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. Expressed only in pancreas and colon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aquaporin8; liver, heart, and placenta water channel; MGC93492; unnamed protein product
Gene Aliases: AQP-8
UniProt ID: (Human) O94778, (Mouse) P56404, (Rat) P56405
Entrez Gene ID: (Human) 343, (Mouse) 11833, (Rat) 29172
water transport
urea transport
methylammonium transport
one-carbon compound transport
B cell differentiation
transepithelial water transport
regulation of cholesterol biosynthetic process
transmembrane transport
cellular response to cAMP
urea transmembrane transport
ammonium transmembrane transport
methylammonium transmembrane transport
hydrogen peroxide transmembrane transport
ammonium import across plasma membrane
cellular detoxification
canalicular bile acid transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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