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Calpain 2 Antibody (PA5-78912) in ICC/IF
Immunocytochemistry analysis of Calpain 2 using anti-Calpain 2 antibody (Product # PA5-78912) . Calpain 2 was detected in a section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Calpain 2 antibody (Product # PA5-78912) overnight at 4°C. DyLight®488 Conjugated ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-78912
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human A431 whole cell, human Hela whole cell, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue. IHC: human intestinal cancer tissue, human lung cancer tissue, rat small intestine tissue. ICC/IF: HELA cell. Flow: U87 cell.
靶标信息
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 80 kDa M-calpain subunit; Calcium-activated neutral proteinase 2; CALP80; Calpain large polypeptide L2; Calpain M-type; Calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; Millimolar-calpain
基因别名: AI326419; CANPL2; Capa-2; Capa2; CAPN2; m-calpain; m-calpin
UniProt ID: (Mouse) O08529, (Rat) Q07009
Entrez Gene ID: (Mouse) 12334, (Rat) 29154
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
