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Invitrogen
FGF21 Polyclonal Antibody
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FIGURE: 1 / 3
FGF21 Antibody (PA5-79254) in WB
Western blot analysis of FGF21 in Lane 1: A549 whole cell lysate, Lane 2: A431 whole cell lysate, Lane 3: HEPA whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with FGF21 polyclonal antibody (Product # PA5-79254) at a dilution of 0.5 µg/mL (overnig... View More
Product Details
PA5-79254
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FGF21 (43-58aa QVRQRYLYTDDAQQTE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A549 whole cell, A431 whole cell, HEPA whole cell, rat spleen tissue.
Target Information
The protein encoded by this gene is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FGF; FGF-21; FGFL; Fibroblast growth factor; Fibroblast growth factor 21; PRO10196
Gene Aliases: FGF21; UNQ3115/PRO10196
UniProt ID: (Human) Q9NSA1, (Mouse) Q9JJN1
Entrez Gene ID: (Human) 26291, (Rat) 170580, (Mouse) 56636
signal transduction
cell-cell signaling
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
regulation of low-density lipoprotein particle clearance
neurogenesis
regulation of cell migration
positive regulation of MAPK cascade
positive regulation of glucose import
positive regulation of ERK1 and ERK2 cascade
positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway
positive regulation of cold-induced thermogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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