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Invitrogen
MCM3 Polyclonal Antibody
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图: 1 / 9
MCM3 Antibody (PA5-79646) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of MCM3 in Hela cell using MCM3 Polyclonal Antibody (Product # PA5-79646). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Tubulin Alpha antibody overnight at 4°C. DyLight 488 conjugated goat anti-rabbit IgG and Cy3 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The se... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79646
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3 (13-27aa REAQRDYLDFLDDEE).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human Raji whole cell, rat thymus tissue, rat C6 whole cell, mouse thymus tissue, mouse NIH/3T3 whole cell. IHC: human cervical cancer tissue, human breast cancer tissue, rat spleen tissue. ICC/IF: Hela cell. Flow: Hela cell.
靶标信息
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA polymerase alpha holoenzyme-associated protein P1; DNA replication licensing factor MCM3; MGC1157; mini chromosome maintenance deficient; minichromosome maintenance deficient 3; P1-MCM3; p102; RLF subunit beta; RP1-108C2.3
基因别名: AL033361; C80350; MCM3; Mcmd; Mcmd3; P1; p1.m
UniProt ID: (Mouse) P25206
Entrez Gene ID: (Mouse) 17215, (Rat) 316273
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
