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Invitrogen
MCM7 Polyclonal Antibody
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MCM7 Antibody (PA5-79651) in ICC/IF
Immunocytochemistry analysis of MCM7 using anti-MCM7 antibody (Product # PA5-79651) . MCM7 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MCM7 antibody (Product # PA5-79651) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79651
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human MCM7 recombinant protein (Position: D526-V719).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell. IHC: Mouse Testis tissue, Rat Intestine tissue, Human Lung Cancer tissue. ICC/IF: U20S cell, A549 cell. Flow: A431 cell.
靶标信息
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDC47 homolog; DNA replication licensing factor MCM7; mini chromosome maintenance deficient 7; minichromosome maintenance deficient 7; minichromosome maintenance protein 7; P1 P85MCM; P1.1-MCM3
基因别名: AI747533; CDC47; mCDC47; MCM2; MCM7; Mcmd7
UniProt ID: (Mouse) Q61881
Entrez Gene ID: (Mouse) 17220, (Rat) 288532
DNA replication
DNA unwinding involved in DNA replication
DNA replication initiation
cellular response to DNA damage stimulus
cell cycle
cell proliferation
regulation of phosphorylation
cellular response to xenobiotic stimulus
regulation of transcription, DNA-templated
response to drug
cellular response to organic substance
cellular response to epidermal growth factor stimulus
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