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Invitrogen
MED4 Polyclonal Antibody
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图: 1 / 7
MED4 Antibody (PA5-79659) in IHC (P)
Immunohistochemistry analysis of MED4 on paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with MED4 polyclonal antibody (Product# PA5-79659) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79659
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human MED4 recombinant protein (Position: R27-K140).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human THP-1 whole cell, human HepG2 whole cell, human HEL whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse Ana-1 whole cell. IHC: mouse brain tissue, mouse small intestine tissue, rat brain tissue, rat small intestine tissue, human mammary cancer tissue, human rectal cancer tissue.
靶标信息
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (Sato et al., 2004).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; FLJ10956; Mediator complex subunit 4; Mediator of RNA polymerase II transcription subunit 4; mediator of RNA polymerase II transcription, subunit 4 homolog; p36 TRAP/SMCC/PC2 subunit; RP11-90M2.2; TRAP/SMCC/PC2 subunit p36 subunit; vitamin D receptor interacting protein; Vitamin D3 receptor-interacting protein complex 36 kDa component
基因别名: 2410046H15Rik; ARC36; DRIP36; HSPC126; MED4; TRAP36; Vdirp; VDRIP
UniProt ID: (Rat) Q561Q8, (Mouse) Q9CQA5
Entrez Gene ID: (Rat) 306030, (Mouse) 67381
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
biological_process
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
positive regulation of transcription, DNA-templated
transcription, DNA-templated
regulation of transcription, DNA-templated
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
