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Invitrogen
COBRA1 Polyclonal Antibody
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COBRA1 Antibody (PA5-79728) in WB
Western blot analysis of COBRA1 in, Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79728
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human COBRA1 recombinant protein (Position: M1-E252).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, mouse kidney tissue, human MDA-MB-231 whole cell.
靶标信息
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2), either NELFC or NELFD (TH1L), and NELFE (RDBP), a heterodimer of SPT4 (SUPT4H1), to cause transcriptional pausing of RNA polymerase II (Narita et al., 2003).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cofactor of BRCA1; DKFZp586B0519; Negative elongation factor B; negative elongation factor complex member B, Cobra1; negative elongation factor protein B; NELF-B; ortholog of human cofactor of BRCA1 (COBRA1); RP13-122B23.3
基因别名: A730008L03Rik; AB041607; AI663983; COBRA1; KIAA1182; MNCb-5210; Nelf-b; NELFB; RGD1307832
UniProt ID: (Mouse) Q8C4Y3
Entrez Gene ID: (Mouse) 58202, (Rat) 311796
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