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Invitrogen
CPI-17 Polyclonal Antibody
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CPI-17 Antibody (PA5-79858) in ICC/IF
Immunocytochemistry analysis of CPI17 alpha using anti-CPI17 alpha antibody (Product # PA5-79858) . CPI17 alpha was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CPI17 alpha antibody (Product # PA5-79858) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was cou... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79858
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue, PANC-1 whole cell. IHC: mouse testis tissue, rat lung tissue, human intestinal cancer tissue, human lung cancer tissue, human placenta tissue. ICC/IF: U20S cell. Flow: U20S cell.
靶标信息
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 17 kDa PKC-potentiated inhibitory protein of PP1; CPI-17; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1 regulatory subunit 14A
基因别名: 1110001M11Rik; Cpi; CPI17; PPP1INL; PPP1R14A
UniProt ID: (Rat) Q99MC0, (Mouse) Q91VC7
Entrez Gene ID: (Rat) 114004, (Mouse) 68458
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