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Invitrogen
PSD Polyclonal Antibody
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FIGURE: 1 / 1
PSD Antibody (PA5-79889) in WB
Western blot analysis of PSD in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with PSD polyclonal antibody (Product # PA5-79889) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat ant... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79889
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human PSD recombinant protein (Position: T536-K803).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue.
Target Information
PSD, which called EFA6A, is 63% identical to EFA6B (PSD4) and contains a Sec7 domain adjacent to a PH domain, followed by a C-terminal coiled-coil motif. This gene is mapped to chromosome 10q24. And it encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: exchange factor for ADP-ribosylation factor guanine nucleotide factor 6; exchange factor for ARF6 A; pleckstrin and Sec7 domain containing homolog; pleckstrin homology and SEC7 domain-containing protein 1; pleckstrin-Sec7 domains-containing; unnamed protein product
Gene Aliases: 1110007H17Rik; A930015K15; EFA6; EFA6A; mKIAA2011; PSD1; Psdl; TYL
UniProt ID: (Human) A5PKW4, (Mouse) Q5DTT2
Entrez Gene ID: (Human) 5662, (Rat) 171381, (Mouse) 73728
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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