Invitrogen

SATB1 Polyclonal Antibody

Name: SATB1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79954
Availability: InStock

高级验证

查看其他22个SATB1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (3)
高级验证 (1)

图: 1 / 4

SATB1 Antibody (PA5-79954) in WB

Western blot was performed using Anti-SATB1 Polyclonal Antibody (Product # PA5-79954) and a ~100kDa band corresponding to SATB1 was observed in Mouse Spleen and Mouse Thymus. An uncharacterized band (*) at ~60 kDa and ~ 50kDa was observed in Mouse Thymus and Rat Spleen tissues respectively. Tissue extracts (30 µg) of Mouse Spleen (Lane 1), Rat Spleen (Lane 2), Mouse Thymus (Lane 3), Mouse Cerebellum (Lane 4) and Mouse Kidney (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SATB1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of SATB1 was observed in Mouse Spleen and Mouse Thymus in comparison to Mouse Cerebellum, Mouse Kidney and Rat Spleen using Anti-CYP2E1 Polyclonal antibody (Product # PA5-79954) in Western Blot. {RE}

Immunogen image

Immunogen Image SATB1 Polyclonal Antibody

产品信息

PA5-79954

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human SATB1 recombinant protein (Position: D638-D763).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2747069

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat thymus tissue, mouse thymus tissue. Flow: PC-3 cell.

靶标信息

SATB1 (DNA-binding protein SATB1) is a crucial silencing factor that contributes to the initiation of X inactiviation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence, and at nuclear matrix- or scaffold-associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB1 is also a transcriptonal repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner by binding to matrix attachment regions of DNA and inducing a local chromatin-loop remodeling. SATB1 modulates the genes that are essential in the maturatio of the immune T-cell CD8SP from thymocytes. Alu-like motifs and SATB1-binding sites provide an unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL1 and IL2RA in T-cells by binding to the same domain as HDAC1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。