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Invitrogen
SCRIB Polyclonal Antibody
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SCRIB Antibody (PA5-79959) in ICC/IF
Immunocytochemistry analysis of SCRIBBLE using anti-SCRIBBLE antibody (Product # PA5-79959) . SCRIBBLE was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SCRIBBLE antibody (Product # PA5-79959) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79959
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human COLO-320 whole cell, human 22RV1 whole cell, human SGC-7901 whole cell. ICC/IF: U20S cell. Flow: A549 cell, HepG2 cell.
靶标信息
SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: circletail; CRIB1; hScrib; KIAA0147; oSCRIB; PDZ-domain protein scribble; Protein LAP4; Protein scribble homolog; S Vartul; SCRB1; SCRIB1; Scribble; scribble homolog 1; scribbled homolog; Vartul
基因别名: AI118201; Crc; CRIB; CRIB1; KIAA0147; LAP4; mKIAA0147; RGD1565055; SCRB1; SCRIB; SCRIB1; VARTUL
UniProt ID: (Mouse) Q80U72
Entrez Gene ID: (Mouse) 105782, (Rat) 362938
neural tube closure
positive regulation of receptor recycling
auditory receptor cell morphogenesis
multicellular organism development
asymmetric protein localization
cell proliferation
synaptic vesicle targeting
morphogenesis of embryonic epithelium
single organismal cell-cell adhesion
cell migration
cochlear nucleus development
cell differentiation
establishment of apical/basal cell polarity
wound healing
positive regulation of apoptotic process
astrocyte cell migration
negative regulation of mitotic cell cycle
synaptic vesicle endocytosis
positive chemotaxis
auditory receptor cell stereocilium organization
apoptotic process involved in morphogenesis
mammary gland duct morphogenesis
protein localization to adherens junction
activation of GTPase activity
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
