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Invitrogen
UCP2 Polyclonal Antibody
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FIGURE: 1 / 4
UCP2 Antibody (PA5-80203) in WB
Western blot analysis of UCP2 in Lane 1: rat spleen tissue lysate, Lane 2: rat cardiac muscle tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: mouse spleen tissue lysate, Lane 5: mouse cardiac muscle tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: human HeLa whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was b... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-80203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human UCP2 (134-170aa AQPTDVVKVRFQAQARAGGGRRYQSTVNAYKTIAREE).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat spleen tissue, rat cardiac muscle tissue, rat brain tissue, mouse spleen tissue, mouse cardiac muscle tissue, mouse brain tissue, human Hela whole cell.
Target Information
UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Dicarboxylate carrier SLC25A8; Dicarboxylate carrier UCP2; Mitochondrial uncoupling protein 2; Solute carrier family 25 member 8; UCP 2; UCPH; uncoupling protein; Uncoupling protein 2, mitochondrial
Gene Aliases: SLC25A8; UCP2
UniProt ID: (Rat) P56500, (Mouse) P70406
Entrez Gene ID: (Rat) 54315, (Mouse) 22228
response to superoxide
response to hypoxia
mitochondrial transport
female pregnancy
aging
response to glucose
positive regulation of cell death
cellular response to insulin stimulus
cellular response to hormone stimulus
cellular response to amino acid starvation
negative regulation of apoptotic process
regulation of mitochondrial membrane potential
negative regulation of insulin secretion involved in cellular response to glucose stimulus
response to fatty acid
cellular response to glucose stimulus
liver regeneration
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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