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Invitrogen
Lamin A Polyclonal Antibody
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FIGURE: 1 / 3
Lamin A Antibody (PA5-81211) in ICC/IF
Immunofluorescence staining of Lamin A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Lamin A Polyclonal Antibody (Product # PA5-81211, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Product Details
PA5-81211
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human LMNA/lamins A
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human LMNA.
Target Information
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 70 kDa lamin; epididymis secretory sperm binding protein; lamin A protein; lamin A/C; lamin A/C-like 1; lamin C; lamin C protein; mandibuloacral dysplasia type A; OTTHUMP00000015843; OTTHUMP00000015846; OTTHUMP00000015847; Prelamin-A/C; progerin; renal carcinoma antigen NY-REN-32; unnamed protein product
Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; PRO1
UniProt ID: (Human) P02545
Entrez Gene ID: (Human) 4000
protein import into nucleus
nucleus organization
nuclear envelope organization
nuclear migration
muscle organ development
protein localization
negative regulation of cell proliferation
positive regulation of gene expression
regulation of cell migration
establishment or maintenance of microtubule cytoskeleton polarity
heterochromatin assembly
regulation of protein stability
regulation of telomere maintenance
protein localization to nucleus
nuclear pore localization
ventricular cardiac muscle cell development
cellular response to hypoxia
negative regulation of mesenchymal cell proliferation
negative regulation of release of cytochrome c from mitochondria
cellular senescence
protein localization to nuclear envelope
regulation of protein localization to nucleus
negative regulation of cardiac muscle hypertrophy in response to stress
DNA double-strand break attachment to nuclear envelope
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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