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Invitrogen
TRIB1 Polyclonal Antibody
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TRIB1 Antibody (PA5-87662) in WB
Western blot analysis of TRIB1 in extracts of various cell lines. Samples were incubated with TRIB1 Polyclonal antibody (Product # PA5-87662) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-87662
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein (or fragment).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
3.35 mg/mL
规格
335 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: LGDLKLRKFV FSTEERTQLR LESLEDTHIM KGEDDALSDK HGCPAYVSPE ILNTTGTYSG KAADVWSLGV MLYTLLVGRY PFHDSDPSAL FSKIRRGQFC IPEHISPKAR CLIRSLLRRE PSERLTAPEI LLHPWFESVL EPGYIDSEIG TSDQIVPEYQ EDSDISSFFC; Positive Samples: U-87MG, BxPC-3, Raji
靶标信息
TRIB1 also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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