Invitrogen

Tyrosinase Polyclonal Antibody

Name: Tyrosinase Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-89322
Availability: InStock

查看其他34个Tyrosinase抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

Tyrosinase Antibody in Western Blot (WB)

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Tyrosinase Antibody (PA5-89322) in WB

Western blot analysis of Tyrosinase in extracts of rat skin. Samples were incubated with Tyrosinase Polyclonal antibody (Product # PA5-89322) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Tyrosinase Polyclonal Antibody

产品信息

PA5-89322

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:1,000-1:5,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 20-340 of human Tyrosinase (NP_0003631)

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.17 mg/mL

117 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2805492

产品详细信息

Immunogen sequence: FPRACVSSKN LMEKECCPPW SGDRSPCGQL SGRGSCQNIL LSNAPLGPQF PFTGVDDRES WPSVFYNRTC QCSGNFMGFN CGNCKFGFWG PNCTERRLLV RRNIFDLSAP EKDKFFAYLT LAKHTISSDY VIPIGTYGQM KNGSTPMFND INIYDLFVWM HYYVSMDALL GGSEIWRDID FAHEAPAFLP WHRLFLLRWE QEIQKLTGDE NFTIPYWDWR DAEKCDICTD EYMGGQHPTN PNLLSPASFF SSWQIVCSRL EEYNSHQSLC NGTPEGPLRR NPGNHDKSRT PRLPSSADVE FCLSLTQYES GSMDKAANFS F; Positive Samples: Rat skin; Cellular Location: Melanosome membrane, Single-pass type I membrane protein

靶标信息

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。