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Invitrogen
SIN3A Polyclonal Antibody
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FIGURE: 1 / 1
SIN3A Antibody (PA5-89535) in WB
Western blot analysis of SIN3A in extracts of 293T cells. Samples were incubated with SIN3A Polyclonal antibody (Product # PA5-89535) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-89535
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Sin3A (NP_0562921)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.19 mg/mL
Amount
19 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MKRRLDDQES PVYAAQQRRI PGSTEAFPHQ HRVLAPAPPV YEAVSETMQS ATGIQYSVTP SYQVSAMPQS SGSHGPAIAA VHSSHHHPTA VQPHGGQVVQ SHAHPAPPVA PVQGQQQFQR; Positive Samples: 293T; Cellular Location: Nucleus, nucleolus
Target Information
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: histone deacetylase complex subunit Sin3a; Mammalian Sin3A; similar to yeast SIN3; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; transcriptional co-repressor Sin3A; transcriptional corepressor Sin3a; transcriptional regulator, SIN3A; unnamed protein product
Gene Aliases: CHR15DELq24; DEL15Q24; WITKOS
UniProt ID: (Human) Q96ST3
Entrez Gene ID: (Human) 25942, (Rat) 363067
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
activation of innate immune response
positive regulation of defense response to virus by host
hematopoietic progenitor cell differentiation
DNA replication
regulation of transcription, DNA-templated
protein localization
regulation of hormone levels
positive regulation of G2/M transition of mitotic cell cycle
cerebral cortex neuron differentiation
regulation of axon extension
heterochromatin assembly
negative regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
response to methylglyoxal
regulation of cell cycle
type I interferon signaling pathway
cellular response to glucose stimulus
cellular response to tert-butyl hydroperoxide
negative regulation of protein localization to nucleus
cellular response to dopamine
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of transcription initiation from RNA polymerase II promoter
negative regulation of stem cell population maintenance
positive regulation of stem cell population maintenance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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