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Invitrogen
DDX3Y Polyclonal Antibody
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DDX3Y Antibody (PA5-90055) in WB
Western blot analysis of DDX3Y in extracts of various cell lines. Samples were incubated with DDX3Y Polyclonal antibody (Product # PA5-90055) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-90055
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_0011161371)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.50 mg/mL
规格
50 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MSHVVVKNDP ELDQQLANLD LNSEKQSGGA STASKGRYIP PHLRNREASK GFHDKDSSGW SCSKDKDAYS SFGSRDSRGK PGYFSERGSG SRGRFDDRGR SDYDGIGNRE RPGFGRFERS GHSRWCDKSV EDDWSKPLPP SERLEQELFS GGNTGINFEK YDDIPVEATG SNCPPHIENF SDIDMGEIIM GNIELTRYTR PTPVQKHAIP; Positive Samples: A-549, HeLa, rat testis; Cellular Location: Cytoplasm, Nucleus
靶标信息
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX3Y is one member of DEAD box RNA helicases, encode by the gene locate on the chromosome Y in the azoospermia factor A region.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
nucleic acid binding
DNA binding
RNA binding
GTPase activity
ATP-dependent DNA helicase activity
ATP-dependent RNA helicase activity
helicase activity
ATP binding
transcription factor binding
poly(A) binding
eukaryotic initiation factor 4E binding
ATPase activity
nucleoside-triphosphatase activity
translation initiation factor binding
RNA stem-loop binding
ribosomal small subunit binding
CTPase activity
poly(A) RNA binding
mRNA 5'-UTR binding
chromosome segregation
metabolic process
extrinsic apoptotic signaling pathway via death domain receptors
response to virus
RNA secondary structure unwinding
positive regulation of gene expression
negative regulation of translation
positive regulation of cell growth
negative regulation of cell growth
negative regulation of protein complex assembly
DNA duplex unwinding
stress granule assembly
intracellular signal transduction
mature ribosome assembly
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of viral genome replication
innate immune response
positive regulation of translation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of translational initiation
cellular response to arsenic-containing substance
cellular response to osmotic stress
intrinsic apoptotic signaling pathway
positive regulation of G1/S transition of mitotic cell cycle
negative regulation of intrinsic apoptotic signaling pathway
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