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Invitrogen
RAD51 Polyclonal Antibody
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FIGURE: 1 / 4
RAD51 Antibody (PA5-92858) in IHC (P)
Immunohistochemistry analysis of RAD51 in paraffin-embedded mouse testis. Samples were incubated with RAD51 Polyclonal antibody (Product # PA5-92858) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-92858
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human Rad51 (NP_0028662)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.81 mg/mL
Amount
81 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-937, Mouse brain, Mouse ovary, Mouse testis, Rat testis; Cellular Location: Cytoplasm, Mitochondrion matrix, Nucleus, centrosome, cytoskeleton, microtubule organizing center, perinuclear region
Immunogen sequence: MAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein RAD51 homolog 1; RAD51 homolog; RAD51 homolog A
Gene Aliases: AV304093; Rad51; Rad51a; Reca; RGD1563603
UniProt ID: (Mouse) Q08297
Entrez Gene ID: (Mouse) 19361, (Rat) 499870
nucleotide binding
DNA binding
chromatin binding
damaged DNA binding
double-stranded DNA binding
single-stranded DNA binding
protein binding
ATP binding
protein C-terminus binding
DNA-dependent ATPase activity
identical protein binding
single-stranded DNA-dependent ATPase activity
DNA polymerase binding
molecular_function
DNA metabolism protein
double-strand break repair via homologous recombination
regulation of protein phosphorylation
DNA metabolic process
DNA unwinding involved in DNA replication
DNA repair
DNA recombination
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
regulation of double-strand break repair via homologous recombination
replication fork processing
telomere organization
positive regulation of DNA ligation
protein homooligomerization
chromosome organization involved in meiotic cell cycle
cellular response to alkaloid
cellular response to ionizing radiation
cellular response to hydroxyurea
cellular response to camptothecin
replication-born double-strand break repair via sister chromatid exchange
response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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