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Invitrogen
GRASP65 Polyclonal Antibody
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图: 1 / 4
GRASP65 Antibody (PA5-93091) in ICC/IF
Immunofluorescence analysis of Golgi reassembly-stacking protein 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GRASP65 Polyclonal Antibody (Product # PA5-93091) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-93091
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GRASP65 (NP_1141051)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.21 mg/mL
规格
21 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: ALPLGAPPPD ALPPGPTPED SPSLETGSRQ SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS SSHERGGEAT WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED GLSAELLEAQ AEEEPASTEG LDTGTEAEGL DSQAQISTTE; Positive Samples: BT474, U-937; Cellular Location: Cytoplasmic side, Golgi apparatus, Peripheral membrane protein, cis-Golgi network membrane
靶标信息
Grasp65 is a structural protein of the golgi apparatus. It has been implicated in the stacking of golgi cisternae. Grasp65 is shown to have the properties to bind surfaces together in a mitotically regulated matter.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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