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Invitrogen
PNK Polyclonal Antibody
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FIGURE: 1 / 5
PNK Antibody (PA5-93150) in ICC/IF
Immunofluorescence analysis of PNK in GFP-RNF168 transgenic U2OS cells. Samples were incubated with PNK Polyclonal antibody (Product # PA5-93150). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-93150
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.68 mg/mL
Amount
68 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RTVAVKQLGV NPSTTGTQEL KPGLEGSLGV GDTLYLVNGL HPLTLRWEET RTPESQPDTP PGTPLVSQDE KRDAELPKKR MRKSNPGWEN LEKLLVFTAA GVKPQGKVAG FDLDGTLITT RSGKVFPTGP SDWRILYPEI PRKLRELEAE GYKLVIFTNQ MSIGRGKLPA EEFKAKVEAV VEKLGVPFQV LVATHAGLYR KPVTGMWDHL QEQANDGTPI SIGDSIFVGD AAGRPANWAP GRKKKDFSCA DRLFALNLGL P; Positive Samples: HL-60, HepG2, Rat liver; Cellular Location: Nucleus
Target Information
Anti-human Polynucleotide Kinase Mouse Monoclonal Antibody recognizes polynucleotide kinase (PNK), a 57 kDa nuclear enzyme implicated in DNA strand repair processes that occur during DNA replication and recombination, or in response to DNA damaging agents. PNK targets the DNA 5'-hydroxy and 3'-phosphate termini for phosphorylation and dephosphorylation events. During strand breakage in human cells, normal PNK associates with other nuclear proteins to prepare the DNA termini for the enzymatic actions of DNA polymerase-beta and DNA ligase III. PNK is a key functional component in maintaining the genetic stability of human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; phosphatase/kinase; PKNP; polynucleotide kinase-3'-phosphatase
Gene Aliases: 1810009G08Rik; PNK
Entrez Gene ID: (Mouse) 59047, (Rat) 308576
DNA repair
cellular response to DNA damage stimulus
response to oxidative stress
metabolic process
phosphorylation
dephosphorylation
DNA damage response, detection of DNA damage
nucleotide phosphorylation
DNA 3' dephosphorylation involved in DNA repair
polynucleotide 3' dephosphorylation
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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