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Invitrogen
MIF Polyclonal Antibody
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图: 1 / 6
MIF Antibody (PA5-94938) in ICC/IF
Immunofluorescence analysis of Macrophage migration inhibitory factor was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MIF Polyclonal Antibody (Product # PA5-94938) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Al... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-94938
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human MIF (98-115aa NYYDMNAANVGWNNSTFA).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human 293T whole cell, rat kidney tissue, rat NRK whole cell, rat PC-12 whole cell, mouse kidney tissue, mouse HBZY-1 whole cell, mouse RAW2647 whole cell. IHC: mouse brain tissue, rat brain tissue. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase
基因别名: GIF; Glif; Mif
UniProt ID: (Mouse) P34884, (Rat) P30904
Entrez Gene ID: (Mouse) 17319, (Rat) 81683
prostaglandin biosynthetic process
positive regulation of protein phosphorylation
brain renin-angiotensin system
immune system process
positive regulation of acute inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
inflammatory response
cell surface receptor signaling pathway
spermatogenesis
cell aging
cell proliferation
negative regulation of gene expression
positive regulation of protein kinase A signaling
positive regulation of smooth muscle cell migration
carboxylic acid metabolic process
DNA damage response, signal transduction by p53 class mediator
positive regulation of B cell proliferation
positive regulation of lipopolysaccharide-mediated signaling pathway
negative regulation of cellular protein metabolic process
negative regulation of myeloid cell apoptotic process
positive regulation of peptidyl-serine phosphorylation
regulation of cell proliferation
positive regulation of phosphorylation
drinking behavior
negative regulation of apoptotic process
positive regulation of potassium ion transport
positive regulation of MAP kinase activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
innate immune response
positive regulation of glycolytic process
positive regulation of glucose import
positive regulation of fibroblast proliferation
positive regulation of axon regeneration
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immune response
positive chemotaxis
positive regulation of prostaglandin secretion involved in immune response
positive regulation of myeloid leukocyte cytokine production involved in immune response
protein homotrimerization
positive regulation of ERK1 and ERK2 cascade
negative regulation of cell cycle arrest
positive regulation of arachidonic acid secretion
negative regulation of cell aging
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
hair follicle development
brain development
aging
metabolic process
response to heat
response to mechanical stimulus
response to hormone
response to inorganic substance
response to estradiol
response to lipopolysaccharide
response to progesterone
response to menaquinone
response to insulin
response to vitamin E
wound healing
response to drug
skin development
response to glucocorticoid
cellular response to hydrogen peroxide
cellular response to hypoxia
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