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Invitrogen
IKAROS Polyclonal Antibody
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图: 1 / 7
IKAROS Antibody (PA5-95293) in ICC/IF
Immunocytochemistry analysis of Ikaros using anti-Ikaros2 antibody (Product # PA5-95293) . Ikaros was detected in a section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-Ikaros antibody (Product # PA5-95293) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with D... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95293
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human Ikaros (428-459aa LKEEHRAYDLLRAASENSQDALRVVSTSGEQM).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Daudi whole cell. IHC: mouse spleen tissue, rat spleen tissue, human tonsil tissue. ICC/IF: MCF-7 cell. Flow: U937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA-binding protein Ikaros; hIk-1
基因别名: 5832432G11Rik; hlk-1; Ikaros; LyF-1; mKIAA4227; RGD1562979; Zfpn1a1; Znfn1a1
Entrez Gene ID: (Mouse) 22778, (Rat) 305501
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
protein heterodimerization activity
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
natural killer cell differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
hemopoiesis
B cell differentiation
T cell differentiation
forebrain development
positive regulation of multicellular organism growth
positive regulation of B cell differentiation
positive regulation of neutrophil differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
lymph node development
thymus development
Peyer's patch development
gland development
positive regulation of NK T cell differentiation
retina development in camera-type eye
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