Invitrogen

RAB9 Polyclonal Antibody

Name: RAB9 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95339
Availability: InStock

查看其他16个RAB9抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (3)

RAB9 Antibody in Immunocytochemistry (ICC/IF)

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RAB9 Antibody (PA5-95339) in ICC/IF

Immunocytochemistry analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (Product # PA5-95339) . Rab9/RAB9A was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-Rab9/RAB9A antibody (Product # PA5-95339) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Th... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image RAB9 Polyclonal Antibody

产品信息

PA5-95339

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9 (156-193aa KDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRK).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807142

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human HepG2 whole cell, human 293T whole cell, monkey COS-7 whole cell, mouse NIH/3T3 whole cell. ICC/IF: Hela cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。