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Invitrogen
DDAH1 Polyclonal Antibody
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图: 1 / 7
DDAH1 Antibody (PA5-95366) in ICC/IF
Immunocytochemistry analysis of DDAH1 using anti-DDAH1 antibody (Product # PA5-95366). DDAH1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DDAH1 antibody (Product # PA5-95366)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vis... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95366
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH1 (195-226aa QKALKIMQQMSDHRYDKLTVPDDIAANCIYLN).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HUVEC whole cell, human HepG2 whole cell, human Caco-2 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue . Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DDAH-1; DDAHI; Dimethylargininase-1; FLJ21264; FLJ25539; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; NG,NG dimethylarginine dimethylaminohydrolase; RP4-621F18.1
基因别名: 2410006N07Rik; 2510015N06Rik; AI987801; AW050362; Ddah; Ddah1
UniProt ID: (Mouse) Q9CWS0, (Rat) O08557
Entrez Gene ID: (Mouse) 69219, (Rat) 64157
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
