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Invitrogen
KDM5B Polyclonal Antibody
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KDM5B Antibody (PA5-95382) in ICC/IF
Immunocytochemistry analysis of KD.M5B using anti-KD.M5B antibody (Product # PA5-95382) . KD.M5B was detected in a section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-KD.M5B antibody (Product # PA5-95382) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DA... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95382
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence in the middle region of human KDM5B (641-685aa DVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFE LL).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: monkey COS-7 whole cell, human SH-SY5Y whole cell, rat testis tissue, mouse testis tissue. IHC: human intestinal cancer tissue, human intestinal cancer tissue, human lung cancer tissue, human breast cancer tissue, mouse testis tissue, rat testis tissue. ICC/IF: MCF-7 cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
KDM5B is a histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. KDM5B demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. It acts as a transcriptional corepressor for FOXG1B and PAX9. It favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, KDM5B may act as a tumor suppressor for melanoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: [histone H3]-trimethyl-L-lysine(4) demethylase 5B; Histone demethylase JARID1B; jumonji, AT rich interactive domain 1B (Rbp2 like); Jumonji, AT rich interactive domain 1B (RBP2-like); Jumonji/ARID domain-containing protein 1B; Lysine-specific demethylase 5B; PLU-1; putative DNA/chromatin binding motif 1
基因别名: 2010009J12Rik; 2210016I17Rik; AW556288; D1Ertd202e; Jarid1b; Kdm5b; Kiaa4034; mKIAA4034; PLU-1; Plu1; PUT1; Rb-Bp2; RBBP2H1A; RGD1565602
UniProt ID: (Mouse) Q80Y84
Entrez Gene ID: (Rat) 304809, (Mouse) 75605
molecular_function
DNA binding
transcription corepressor activity
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-trimethyl-K4 specific)
histone demethylase activity (H3-dimethyl-K4 specific)
sulfonate dioxygenase activity
protein binding
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
metal ion binding
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
histone modifying enzyme
histone H3-K4 demethylation
histone H3-K4 demethylation, trimethyl-H3-K4-specific
cellular response to fibroblast growth factor stimulus
negative regulation of transcription, DNA-templated
oxidation-reduction process
response to fungicide
lens fiber cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin modification
regulation of circadian rhythm
rhythmic process
negative regulation of nucleic acid-templated transcription
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