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Invitrogen
CHX10 Polyclonal Antibody
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CHX10 Antibody (PA5-95472) in WB
Western blot analysis of CHX10 in Lane 1: mouse eye ball tissue lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with CHX10 polyclonal antibody (Product # PA5-95472) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95472
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human CHX10 recombinant protein (Position: A249-A361).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Human CHX10 shares 90.3% amino acid (aa) sequence identity with mouse CHX10.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse eye ball tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
CHX10 encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C. elegans ceh-10 homeo domain containing homolog; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10; ocular retardation; Visual system homeobox 2
基因别名: Chx10; Hox-10; Hox10; or; Vsx2
UniProt ID: (Mouse) Q61412
Entrez Gene ID: (Mouse) 12677
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
positive regulation of cell proliferation
negative regulation of cell proliferation
camera-type eye development
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
retinal bipolar neuron differentiation
retina morphogenesis in camera-type eye
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