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Invitrogen
STUB1 Polyclonal Antibody
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图: 1 / 6
STUB1 Antibody (PA5-95507) in ICC/IF
Immunocytochemistry analysis of STUB1 using anti-STUB1 antibody (Product # PA5-95507). STUB1 was detected in a section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-STUB1 antibody (Product # PA5-95507) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-95507
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human STUB1 recombinant protein (Position: R51-Y303).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human MCF-7 whole cell, human Hela whole cell, human THP-1 whole cell, rat pancreas tissue, mouse pancreas tissue. IHC: human colon cancer tissue, human lung cancer tissue, human mammary cancer tissue. ICC/IF: MCF7 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Carboxy terminus of Hsp70-interacting protein; E3 ubiquitin-protein ligase CHIP; LA16c-313D11.6; RING-type E3 ubiquitin transferase CHIP; STIP1 homology and U box-containing protein 1
基因别名: 0610033N24Rik; 2210017D18Rik; 2310040B03Rik; AW046544; Chip; Stub1
UniProt ID: (Mouse) Q9WUD1, (Rat) A6HD62
Entrez Gene ID: (Mouse) 56424, (Rat) 287155
G-protein coupled receptor binding
ubiquitin-protein transferase activity
protein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
enzyme binding
kinase binding
Hsp70 protein binding
protein binding, bridging
TPR domain binding
heat shock protein binding
ubiquitin protein ligase binding
ubiquitin-ubiquitin ligase activity
protein homodimerization activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
SMAD binding
misfolded protein binding
Hsp90 protein binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein polyubiquitination
DNA repair
protein folding
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
cellular response to DNA damage stimulus
protein ubiquitination
ubiquitin-dependent SMAD protein catabolic process
endoplasmic reticulum unfolded protein response
positive regulation of protein ubiquitination
regulation of glucocorticoid metabolic process
negative regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of ubiquitin-protein transferase activity
protein maturation
protein autoubiquitination
protein K63-linked ubiquitination
cellular response to misfolded protein
positive regulation of chaperone-mediated protein complex assembly
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