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Invitrogen
PITX2 Polyclonal Antibody
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FIGURE: 1 / 1
PITX2 Antibody (PA5-95968) in WB
Western blot analysis of PITX2 in extracts of various cell lines. Samples were incubated with PITX2 Polyclonal antibody (Product # PA5-95968) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95968
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MNCMKGPLHL EHRAAGTKLS AVSSSSCHHP QPLAMASVLA PGQPRSLDSS KHRLEVHTIS DTSSPEAAEK DKSQQGKNED VGAEDPSKKK RQRRQRTHFT; Positive Samples: NCI-H490, HeLa, Mouse brain, Rat brain ; Cellular Location: Nucleus
Target Information
Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: all1-responsive gene 1; ALL1-responsive protein ARP1; Bicoid-related homeobox protein 1; BRX1 homeoprotein; Homeobox protein PITX2; homeodomain transcription factor 2; isoform a; isoform b; isoform c; Orthodenticle-like homeobox 2; Paired-like homeodomain transcription factor 2; paired-like homeodomain transcription factor Munc 30; Pituitary homeobox 2; RIEG bicoid-related homeobox transcription factor; rieg bicoid-related homeobox transcription factor 1; rPtx2; S a 1 glycoprotein; S a1glycoprotein; S alpha 1 glycoprotein; S α 1 glycoprotein; S α1glycoprotein; Solurshin; unnamed protein product
Gene Aliases: 9430085M16Rik; ARP1; ASGD4; Brx1; Brx1b; IDG2; IGDS; IGDS2; IHG2; IRID2; Munc30; Otlx2; Pitx-2; PITX2; Pitx2c; PTX2; RGS; RIEG; RIEG1; RS
UniProt ID: (Rat) Q9R0W1, (Mouse) P97474
Entrez Gene ID: (Human) 5308, (Rat) 54284, (Mouse) 18741
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
chromatin DNA binding
identical protein binding
protein homodimerization activity
ribonucleoprotein complex binding
sequence-specific DNA binding
phosphoprotein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
transcriptional repressor activity, RNA polymerase II transcription factor binding
transcription factor binding
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
outflow tract morphogenesis
cardiac neural crest cell migration involved in outflow tract morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
determination of left/right symmetry
positive regulation of cell proliferation
anatomical structure morphogenesis
organ morphogenesis
hair cell differentiation
deltoid tuberosity development
odontogenesis
camera-type eye development
positive regulation of transcription from RNA polymerase II promoter
spleen development
system development
somatotropin secreting cell differentiation
prolactin secreting cell differentiation
embryonic heart tube left/right pattern formation
iris morphogenesis
left/right axis specification
patterning of blood vessels
vasculogenesis
in utero embryonic development
neuron migration
extraocular skeletal muscle development
atrioventricular valve development
pulmonary myocardium development
transcription from RNA polymerase II promoter
brain development
heart development
skeletal muscle tissue development
myoblast fusion
male gonad development
female gonad development
response to hormone
Wnt signaling pathway
subthalamic nucleus development
hypothalamus cell migration
pituitary gland development
neuron differentiation
lung development
regulation of cell migration
embryonic camera-type eye development
response to vitamin A
embryonic hindlimb morphogenesis
vascular smooth muscle cell differentiation
regulation of cell proliferation
odontogenesis of dentin-containing tooth
positive regulation of DNA binding
positive regulation of transcription, DNA-templated
embryonic digestive tract morphogenesis
cardiac muscle tissue development
cardiac muscle cell differentiation
atrial cardiac muscle tissue morphogenesis
ventricular cardiac muscle cell development
digestive system development
ventricular septum morphogenesis
left lung morphogenesis
pulmonary vein morphogenesis
superior vena cava morphogenesis
endodermal digestive tract morphogenesis
cell proliferation involved in outflow tract morphogenesis
positive regulation of myoblast proliferation
multicellular organism development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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