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Invitrogen
SMYD1 Polyclonal Antibody
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FIGURE: 1 / 1
SMYD1 Antibody (PA5-96630) in WB
Western blot analysis of SMYD1 in extracts of various cell lines. Samples were incubated with SMYD1 Polyclonal antibody (Product # PA5-96630) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96630
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SMYD1 (NP_9380151)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.39 mg/mL
Amount
39 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MTIGRMENVE VFTAEGKGRG LKATKEFWAA DIIFAERAYS AVVFDSLVNF VCHTCFKRQE KLHRCGQCKF AHYCDRTCQK DAWLNHKNEC SAIKRYGKVP NENIRLAARI MWRVEREGTG LTEGCLVSVD DLQNHVEHFG EEEQKDLRVD VDTFLQYWPP QSQQFSMQYI SHIFGVINCN GFTLSDQRGL QAVGVGIFPN LGLVNHDCWP NCTVIFNNGN HEAVKSMFHT QMRIELRALG KISEGEELTV SYIDFLNVSE ERKRQLKKQY YFDCTCEHCQ KKLKDDLFLG VKDNPKPSQE VVKEMIQFSK DTLEKIDKAR SEGLYHEVVK LCRECLEKQE PVFADTNIYM; Positive Samples: HepG2, Raji, MCF7, Mouse skeletal muscle, Rat heart, Rat skeletal muscle; Cellular Location: Cytoplasm, Nucleus
Target Information
Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8b-opposite; CD8beta opposite strand; Histone-lysine N-methyltransferase Smyd1; m-BOP; SET and MYND domain-containing protein 1; Zinc finger protein BOP
Gene Aliases: 4632404M21Rik; Bop; C78565; Smyd1; Zmynd18
UniProt ID: (Mouse) P97443
Entrez Gene ID: (Mouse) 12180, (Rat) 297333
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
heart development
positive regulation of myotube differentiation
methylation
skeletal muscle cell differentiation
positive regulation of myoblast differentiation
negative regulation of transcription, DNA-templated
biological_process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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