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Invitrogen
DDIT4 Polyclonal Antibody
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DDIT4 Antibody (PA5-96924) in WB
Western blot analysis of DDIT4 in extracts of various cell lines. Samples were incubated with DDIT4 Polyclonal antibody (Product # PA5-96924) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-96924
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_0619311)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.72 mg/mL
规格
72 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC; Positive Samples: A-549, K-562, DU145, MCF-7; Cellular Location: Cytoplasm, Mitochondrion, cytosol
靶标信息
DDIT4 gene ontology annotations related to this gene include cellular response to dexamethasone stimulus; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of glycolytic process; negative regulation of TOR signaling; protein-containing complex disassembly; reactive oxygen species metabolic process; response to hypoxia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: RP11-442H21.1
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