Invitrogen

IL-33 Polyclonal Antibody

Name: IL-33 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-97869
Availability: InStock

查看其他43个IL-33抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

图: 1 / 1

IL-33 Antibody (PA5-97869) in WB

Western Blot analysis of IL-33 using a IL-33 Polyclonal antibody (Product # PA5-97869) at a concentration of 2 µg/mL. Lane 1: Hela whole cell lysate. Lane 2: Mouse lung tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 32 kDa.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

PA5-97869

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human, Mouse, Pig

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Pig Interleukin-33 protein (123-276AA)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

4.5 mg/mL

100 µg

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

AB_2812483

靶标信息

IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: growth stimulation-expressed; H-IL-33; homolog of mouse growth stimulation-expressed; IL-33; ILN; Interleukin; interleukin 1 receptor-related protein; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin-33 receptor alpha chain; Interleukin33; Lymphocyte antigen 84; nuclear factor from high endothelial venules; Protein ST2; Protein T1; RP11-575C20.2; soluble protein receptor; suppressor of tumorigenicity 2; unnamed protein product

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基因别名: 9230117N10Rik; DER4; FIT-1; IL-33; Il1f11; IL1RL1; Il33; IL33R; Ly84; NF-HEV; ST2; St2-rs1; ST2L; ST2V; Ste2; T1; T1/ST2

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UniProt ID: (Mouse) P14719, (Mouse) Q8BVZ5