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Invitrogen
Phospho-C-RAF (Ser301) Polyclonal Antibody
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Phospho-C-RAF (Ser301) Antibody (PA5-99141) in IHC (P)
Immunohistochemistry analysis of Phospho-C-RAF (Ser301) in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-C-RAF (Ser301) polyclonal antibody (Product # PA5-99141) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-99141
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RAF1(Accession P04049), corresponding to amino acid residues around phosphorylated Ser301.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of C-RAF only when phosphorylated at Sersine 301.
靶标信息
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-RAF; cRaf; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; sarcoma 3611 oncogene; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; vraf1 murine le
基因别名: 6430402F14Rik; AA990557; BB129353; c-Raf; Craf; Craf1; D830050J10Rik; RAF; Raf-1; RAF1; v-Raf
UniProt ID: (Mouse) Q99N57, (Rat) P11345
Entrez Gene ID: (Mouse) 110157, (Rat) 24703
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
receptor signaling protein activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Ras GTPase binding
mitogen-activated protein kinase kinase binding
identical protein binding
metal ion binding
protein heterodimerization activity
small GTPase binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
response to hypoxia
cellular glucose homeostasis
positive regulation of protein phosphorylation
protein phosphorylation
signal transduction
negative regulation of cell proliferation
phosphorylation
cell differentiation
negative regulation of protein complex assembly
positive regulation of peptidyl-serine phosphorylation
somatic stem cell population maintenance
intracellular signal transduction
insulin secretion involved in cellular response to glucose stimulus
response to muscle stretch
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
positive regulation of transcription from RNA polymerase II promoter
neurotrophin TRK receptor signaling pathway
death-inducing signaling complex assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
heart development
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