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Invitrogen
E2F4 Polyclonal Antibody
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图: 1 / 15
E2F4 Antibody (PA5-99166) in ICC/IF
Immunocytochemistry analysis of E2F4 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with E2F4 polyclonal antibody (Product # PA5-99166) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-99166
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human E2F4(Accession Q16254), corresponding to amino acid residues V30-Q80.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total E2F4.
靶标信息
E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa 411-416, varies due to a trinucleotide repeat).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; transcription factor; Transcription factor E2F4
基因别名: 2010111M04Rik; AI427446; E2F-4; E2F4
UniProt ID: (Mouse) Q8R0K9
Entrez Gene ID: (Human) 1874, (Rat) 100360427, (Mouse) 104394
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein domain specific binding
protein dimerization activity
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
transcription factor binding
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
motile cilium assembly
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
centriole assembly
multi-ciliated epithelial cell differentiation
regulation of transcription involved in G1/S transition of mitotic cell cycle
epithelial cell development
transcription, DNA-templated
transcription from RNA polymerase II promoter
cell volume homeostasis
blood circulation
regulation of cell size
organ morphogenesis
regulation of cell proliferation
cilium assembly
mitotic cell cycle
cell cycle
cell projection organization
regulation of cell cycle
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