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Invitrogen

Caspase 1 p20 (Cleaved Asp296) Polyclonal Antibody

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24 篇参考文献

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产品信息

PA5-99390

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
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免疫组化 (IHC)

-
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免疫组化（石蜡） (IHC (P))

1:50-1:200
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免疫组化（冰冻） (IHC (F))

-
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免疫细胞化学 (ICC/IF)

-
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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues I280-V299.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

规格

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2818323

产品详细信息

Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Asp296.

靶标信息

Caspase-1 p20 (cleaved Asp296) refers to the large catalytic subunit generated during activation of pro-caspase-1, an inflammatory cysteine protease encoded by the CASP1 gene. Caspase-1 is synthesized as an inactive zymogen and becomes activated within multiprotein inflammasome complexes in response to infection, cellular stress, or danger-associated signals. Activation involves autoproteolytic cleavage at specific aspartic acid residues, including at Asp296, producing the p20 (large) and p10 (small) subunits that assemble into the active heterotetrameric enzyme. The p20 fragment contains part of the catalytic domain and is commonly used as a marker of caspase-1 activation. Active caspase-1 mediates the proteolytic maturation of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-18 (IL-18) and drives pyroptosis, an inflammatory form of programmed cell death. Detection of cleaved caspase-1 p20 (Asp296) is therefore widely used to assess inflammasome activation in immunology and inflammation research.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CASP-1; CASP1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1; Caspase-1 (Asp296 cleaved); Caspase-1 (cleaved Asp296); Cleaved Caspase-1 p20; cysteine protease; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; unnamed protein product

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基因别名: CASP1; ICE; IL1BC; IL1BCE; P45

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UniProt ID: (Human) P29466, (Mouse) P29452

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Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166

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功能
endopeptidase activity cysteine-type endopeptidase activity protein binding peptidase activity cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process hydrolase activity enzyme binding kinase binding cytokine binding identical protein binding CARD domain binding scaffold protein binding protease
参与通路
negative regulation of transcription from RNA polymerase II promoter plasma membrane repair pattern recognition receptor signaling pathway proteolysis apoptotic process inflammatory response signal transduction osmosensory signaling pathway protein secretion protein autoprocessing protein catabolic process response to lipopolysaccharide positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production interleukin-18-mediated signaling pathway defense response to bacterium regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling icosanoid biosynthetic process ceramide biosynthetic process regulation of inflammatory response positive regulation of inflammatory response protein maturation defense response to virus protein poly-ADP-ribosylation pyroptosis interleukin-1-mediated signaling pathway cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to interferon-gamma cytokine precursor processing signaling receptor ligand precursor processing AIM2 inflammasome complex assembly pyroptotic cell death positive regulation of tumor necrosis factor-mediated signaling pathway response to hypoxia microglial cell activation myoblast fusion memory response to bacterium regulation of autophagy response to organic cyclic compound protein processing interleukin-1 beta production response to ATP response to drug positive regulation of apoptotic process positive regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of cytokine secretion positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion mitochondrial depolarization membrane hyperpolarization cellular response to organic substance programmed necrotic cell death toxin transport midgut development response to toxic substance lung development execution phase of apoptosis
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