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Invitrogen
SMAD5 Polyclonal Antibody
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FIGURE: 1 / 3
SMAD5 Antibody (PA5-99396) in ICC/IF
Immunofluorescent analysis of SMAD5 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SMAD5 polyclonal antibody (Product # PA5-99396) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99396
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SMAD5(Accession Q99717), corresponding to amino acid residues P224-I274.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Smad5.
Target Information
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781C1895; DKFZp781O1323; Dwarfin-C; Dwf-C; hSmad5; JV5-1; MAD homolog 5; Mothers against decapentaplegic homolog 5; mothers against DPP homolog 5; mSmad5; OTTHUMP00000223331; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5
Gene Aliases: 1110051M15Rik; AI451355; Dwf-C; MADH5; Msmad5; MusMLP; SMAD5
UniProt ID: (Mouse) P97454, (Rat) Q9R1V3
Entrez Gene ID: (Mouse) 17129, (Rat) 59328
angiogenesis
Mullerian duct regression
osteoblast fate commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
transforming growth factor beta receptor signaling pathway
multicellular organism development
germ cell development
cell differentiation
erythrocyte differentiation
BMP signaling pathway
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle contraction
bone development
cellular response to organic cyclic compound
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
ossification
ureteric bud development
embryonic pattern specification
regulation of ossification
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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