Search
Invitrogen
Caspase 8 (Cleaved Asp384) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 5
Caspase 8 (Cleaved Asp384) Antibody (PA5-99435) in ICC/IF
Immunocytochemistry analysis of Caspase 8 (Cleaved Asp384) in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Caspase 8 (Cleaved Asp384) polyclonal antibody (Product # PA5-99435) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-99435
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CASP8(Accession Q14790), corresponding to amino acid residues K367-P394.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384.
靶标信息
Caspase-8 (cleaved Asp384) refers to the activated form of caspase-8 generated following proteolytic cleavage at aspartic acid residue 384, a key step in the activation of this initiator caspase in the extrinsic apoptosis pathway. Caspase-8 is encoded by the CASP8 gene and is synthesized as an inactive zymogen (pro-caspase-8) containing tandem death effector domains (DEDs) that enable recruitment to death-inducing signaling complexes (DISCs) formed downstream of death receptors such as Fas (CD95), TRAIL receptors, and TNF receptor 1. Upon DISC assembly, pro-caspase-8 undergoes dimerization and autocatalytic cleavage at specific aspartic acid sites, including Asp384, producing active subunits that assemble into the functional enzyme. Activated caspase-8 initiates the apoptotic cascade by cleaving and activating downstream effector caspases such as caspase-3 and caspase-7, and can also process BID to link extrinsic and intrinsic apoptosis pathways. Detection of cleaved caspase-8 (Asp384) is widely used as a marker of death receptor-mediated apoptotic signaling in cell death and cancer research.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8; Caspase-8 (Asp384 cleaved); caspase-8s; Cleaved Caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog
基因别名: ALPS2B; CAP4; Casp-8; CASP8; FLICE; MACH; MCH5
Entrez Gene ID: (Human) 841
endopeptidase activity
cysteine-type endopeptidase activity
death receptor binding
protein binding
peptidase activity
cysteine-type peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
ubiquitin protein ligase binding
death effector domain binding
identical protein binding
macromolecular complex binding
scaffold protein binding
angiogenesis
regulation of cytokine production
regulation of immune system process
proteolysis
apoptotic process
heart development
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of signal transduction
regulation of tumor necrosis factor-mediated signaling pathway
protein processing
natural killer cell activation
cell differentiation
protein catabolic process
macrophage differentiation
positive regulation of cell migration
response to cobalt ion
response to estradiol
response to lipopolysaccharide
positive regulation of interleukin-1 beta production
response to tumor necrosis factor
TRAIL-activated apoptotic signaling pathway
T cell activation
B cell activation
regulation of apoptotic process
chordate embryonic development
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
regulation of innate immune response
response to ethanol
positive regulation of macrophage differentiation
positive regulation of proteolysis
animal organ development
protein maturation
negative regulation of necroptotic process
syncytiotrophoblast cell differentiation involved in labyrinthine layer development
pyroptosis
cellular response to mechanical stimulus
death-inducing signaling complex assembly
response to anesthetic
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
execution phase of apoptosis
self proteolysis
positive regulation of execution phase of apoptosis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
