Invitrogen

Phospho-EIF4B (Ser422) Polyclonal Antibody

Name: Phospho-EIF4B (Ser422) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-99472
Availability: InStock

查看其他26个eIF4B抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

Phospho-EIF4B (Ser422) Antibody in Western Blot (WB)

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Phospho-EIF4B (Ser422) Antibody (PA5-99472) in WB

Western blot analysis of Phospho-EIF4B (Ser422) in HepG2 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-EIF4B (Ser422) polyclonal antibody (Product # PA5-99472).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Phospho-EIF4B (Ser422) Polyclonal Antibody

产品信息

PA5-99472

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human EIF4B(Accession P23588), corresponding to amino acid residues around phosphorylated Ser422.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2818405

产品详细信息

Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.

靶标信息

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

仅用于科研。不用于诊断过程。未经明确授权不得转售。