Invitrogen

SH3BGR Polyclonal Antibody

4个已发表图片

1 篇参考文献

查看其他5个SH3BGR抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (1)
已发表图片 (4)

SH3BGR Antibody in Western Blot (WB)

SH3BGR Antibody in Western Blot (WB)

图: 1 / 5

SH3BGR Antibody (PA5-99717) in WB

Western blot analysis of SH3BGR in mouse heart tissue lysate (left lane: treated with blocking peptide). Samples were incubated with SH3BGR polyclonal antibody (Product # PA5-99717).

SH3BGR Antibody in Western Blot (WB)

SH3BGR Antibody in Western Blot (WB)

SH3BGR Antibody in Western Blot (WB)

SH3BGR Antibody in Western Blot (WB)

SH3BGR Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SH3BGR Antibody in Western Blot (WB) — Western blot analysis of SH3BGR in mouse heart tissue lysate (left lane: treated with blocking peptide). Samples were incubated with SH3BGR polyclonal antibody (Product # PA5-99717).

SH3BGR Antibody in Western Blot (WB) — Fig. 5 METTL3 regulated the expression of SH3BGR in AC16 cells. A Depletion of METTL3 decreased abundance of m6A-modified SH3BGR mRNA in AC16 cells. B Overexpression of METTL3 induced SH3BGR mRNA m6A modification in AC16 cells. C RT-qPCR analysis of SH3BGR in control and METTL3 knockdown AC16 cells. D Western blotting analysis of METTL3 and SH3BGR with or without METTL3 knockdown in AC16 cells. E , F Statistically analysis of protein expression detected in ( D ). G Volcano plot showing log2 fold change of gene expression and corresponding adjust p-value in METTL3 knockdown compared with control AC16 cells. H RNA-seq data showed increased expression of SH3BGR in METTL3 knockdown AC16 cells. I GO analysis of differentially expressed genes in METTL3 knockdown compared with control AC16 cells. J KEGG analysis of differentially expressed genes in METTL3 knockdown compared with control AC16 cells.

SH3BGR Antibody in Western Blot (WB) — Fig. 8 METTL3 targets SH3BGR affecting cell apoptosis in cKO mice. A Sh3bgr protein level was analyzed by western blotting in the cardiac tissue of cKO and control mice. Gapdh served as an internal control. B Volcano plot showing log2 fold change of gene expression and corresponding adjust p-value in cKO compared with control mice. C GO analysis of differentially expressed genes in the cardiac tissue of cKO compared with controls. D GSEA analysis revealed up-regulated pathway in striated muscle cell apoptotic process. E Heatmap for striated muscle cell apoptotic process. F Western blotting analysis examined the expression of Sh3bgr and Caspase 3 in the hearts from cKO and control mice. beta-Actin served as an internal control.

SH3BGR Antibody in Western Blot (WB) — Fig. 7 METTL3-mediated regulation of SH3BGR expression influencing apoptosis. A Cell apoptosis was examined by flow cytometry in METTL3 knockdown or both METTL3 and SH3BGR knockdown AC16 cells. B Statistically analysis of ( A ). C Western blotting detected the expression of SH3BGR in METTL3 knockdown or both METTL3 and SH3BGR knockdown AC16 cells. -1, -2, -3 represents three different METTL3 siRNAs. GAPDH served as an internal control. D GSEA analysis revealed down-regulated pathway in regulation of intrinsic apoptotic signaling pathway in double knockdown AC16 cells compared to control cells. E Heatmap for intrinsic apoptotic signaling pathway.

SH3BGR Antibody in Western Blot (WB) — The expression of SH3BGR is dysregulated in DS cardiac tissues. A RNA-seq data showed increased expression of SH3BGR in DS cardiac tissues. B RT-qPCR analysis confirmed that SH3BGR mRNA was increased in DS tissues. C , D SH3BGR protein level was analyzed by western blotting in DS and control samples. Data were presented as mean +- SD of three independent experiments. ** p < 0.01 compared with the control group. E RNA-seq data showed upregulated expression of SH3BGR in heart tissues of Dp16 mice.

Immunogen image

SH3BGR Polyclonal Antibody

Immunogen Image SH3BGR Polyclonal Antibody

产品信息

PA5-99717

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SH3BGR(Accession P55822), corresponding to amino acid residues E85-E135.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total SH3BGR.

靶标信息

SH3BGR is a protein coding gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 21-GARP; 21-glutamic acid-rich protein; SH3 domain-binding glutamic acid-rich protein; SH3BGR protein

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基因别名: 5430437A18Rik; SH3BGR

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UniProt ID: (Mouse) Q9WUZ7

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Entrez Gene ID: (Mouse) 50795

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molecular_function SH3 domain binding

biological_process