Power Blotter Cassette - FAQs

当Thermo Scientific Power Blotter与Towbin转膜缓冲液结合使用时，可在45–60分钟内同时转印1-4块小型凝胶或1-2块中型凝胶。请注意，同时转印的凝胶必须配方相同。

当Thermo Scientific Power Blotter与Thermo Scientific一步转膜缓冲液结合使用时，可在5-10分钟内同时转印1-4块小型凝胶或1-2块中型凝胶。请注意，同时转印的凝胶必须配方相同。

Thermo Scientific Power Blotter（货号22834）由带有激活版印迹软件的Thermo Scientific Power Station（货号22838）和Thermo Scientific Power Blot Cassette（货号22835）组成。该系统专用于将蛋白质从聚丙烯酰胺凝胶上快速半干转印到硝化纤维素膜或PVDF膜上。传统的免疫印迹分析技术需要转印1小时至过夜，才能获得良好的转印结果。与Thermo Scientific一步转膜缓冲液结合使用时，Thermo Scientific Power Blotter只需5-10分钟即可带来等于或优于传统转印技术的转印效率，并且无需预平衡凝胶。这种蛋白质转印时间的显著缩短，是通过优化转膜缓冲液的离子强度和增加通过转印膜组的电流[amps (A)/cm2]实现的。该系统已经过验证，对常用的预制胶和自制SDS-PAGE凝胶均适用。Thermo Scientific Power Blotter也可与Towbin缓冲液一起用于标准半干转实验方案。

The Power Blotter System (Cat. No. PB0012) comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter Cassette (Cat. No. PB0002). The Power Blotter System can transfer one midi or up to two mini gels at the same time. The Power Blotter XL System (Cat. No. PB0013) on the other hand comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter XL Cassette (Cat. No. PB0003). The Power Blotter XL System can transfer up to two midi or four mini gels at the same time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Even though the Power Blotter System/Power Blotter XL System does not have the staining program, this can be introduced. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The top (cathode) is stainless steel and the bottom (anode) is platinum-coated stainless steel.

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Yes, you may purchase the Universal Cable Kit (Cat. No. 4377117).

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Here are the SKUs for the power cord. Please order the one appropriate for your region:

- Cat. No. 84858: Power Cord with C13 Connector, North America
- Cat. No. 84859: Power Cord with C13 Connector, Continental Europe and South Korea
- Cat. No. 84860: Power Cord with C13 Connector, United Kingdom and Singapore
- Cat. No. 84855: Chinese 10 Amp Cordset w/ C13 Connector
- Cat. No. 84856: Japanese 15 Amp Cordset w/ C13 Connector
- Cat. No. 84857: Australian 10 Amp Cordset w/ C13 Connector

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes, Power Blotter Select Transfer Stacks are compatible with immunofluorescence detection.

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Yes, both PVDF and nitrocellulose membranes are compatible with the Power Blotter System/Power Blotter XL System. You can use 0.2 µm and 0.45 µm pore size membranes. Our Power Blotter Pre-cut Membranes and Filters (PVDF and nitrocellulose) come with 0.45 µm pore size membranes. Our Power Blotter Select Transfer Stacks (PVDF and nitrocellulose) come with 0.2 µm pore size membranes.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This may be due to salt deposited on moving parts inside the cassette. We recommend rinsing the cassette top and bottom under warm water while removing any sticky salt residue with a gloved hand. Briefly rinse with deionized water and place in a rack to dry. For more thorough cleaning, immerse the unassembled cassette in warm water and use a gloved hand or clean sponge to remove any sticky salt residue. Rinse with deionized water and place perpendicular in a rack to dry.
Note: Failure to keep the cassette top (cathode) and bottom (anode) clean will result in the sticking of moving parts and lead to poor transfer efficiency.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes. You may use the same method that was used with the G2 Fast Blotter: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/transfer-emsa-gels-using-g2-fast-blotter.html

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

No, revalidation is not necessary. The firmware is the same and the blotting efficiency is the same.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

You can use your own consumables (membrane, filter paper with 2.48 mm thickness, and buffer) for standard semi-dry transfer on the Power Blotter System/Power Blotter XL System following the parameters recommended on page 20 of the Power Blotter System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017053-PowerBlotterSystem-UG.pdf).

For fast semi-dry transfer on the Power Blotter System/Power Blotter XL System, you can use your own membrane and filter paper (with 1.8 mm thickness), however, the Power Blotter 1-Step Transfer Buffer or the Pierce 1-Step Transfer Buffer would need to be used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes, but you need to ensure that the total thickness of the filter paper stack on either side of the gel/membrane does not exceed 1.8 mm.

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Yes, utilization of the Power Blotter 1-Step Transfer Buffer (5X) allows fast transfer (within 5 min for low MW proteins/within 7 min for mixed range MW proteins/within 10 min for high MW proteins). The Power Blotter can also be used with the Pierce 1-Step Transfer Buffer (Cat. No. 84731, 84731X5, or 84742) for fast transfer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes, the Power Blotter System/Power Blotter XL System is an open system and you can use your own transfer buffer. However, keep in mind that your own transfer buffer will result in a prolonged transfer time (˜60 min), whereas a fast transfer (5-10 min) can be achieved using the Power Blotter 1-Step Transfer Buffer (5X) or the Pierce 1-Step Transfer Buffer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes, you can order the Power Blotter Cassette XL (Cat. No. PB0003).
Note: The Power Blotter Cassette (Cat. No. PB0002) is not compatible with the G2 Fast Blotter.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Power Blotter Cassette (Cat. No. PB0002) is designed to blot 1-2 mini gels at a time or 1 midi gel and the Power Blotter Cassette XL (Cat. No. PB0003) is designed to blot 1-4 mini gels or 1-2 midi gels at a time. Both cassettes can be used with the Power Blotter. On the other hand, the traditional G2 Fast Blotter is only compatible with the Power Blotter Cassette XL (Cat. No. PB0003); the Power Blotter Cassette (Cat. No. PB0002) will not work with the G2 Fast Blotter.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

You can purchase the Power Blotter Station (Cat. No. PB0010) and the G2 Fast Blotter cassette is compatible with it. The new Power Blotter System is designed for Western transfer alone and does not have a staining module; therefore, the G2 Fast Blotter staining cassette cannot be used on the Power Blotter Station.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We do not offer a staining cassette for the Power Blotter System/Power Blotter XL System but it can be programmed to stain gels. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

No, 1-2 mini gels and 1, 2, or 4 mini gels can be transferred at the same time with the same transfer efficiency using the Power Blotter System and Power Blotter XL System, respectively.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This is most likely due to inefficient binding of some low-molecular weight proteins (<25 kDa) to PVDF membrane. We recommend combining ethanol and Thermo Scientific 1-Step Transfer Buffer in a 15:85 ratio before equilibrating filter paper and membrane (e.g., 15 mL of ethanol with 85 mL of Thermo Scientific 1-Step Transfer Buffer).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are possible causes and solutions:

- Membrane or filter paper was insufficiently equilibrated in Thermo Scientific 1-Step Transfer Buffer: Equilibrate membrane and filter paper in Thermo Scientific 1-Step Transfer Buffer before transfer for a minimum of 5 minutes. Use a sufficient amount of buffer for the equilibration step.
- Insufficient transfer time: Increase transfer time from 7-10 minutes to 10-12 minutes.
- PVDF membrane was not pre-wetted with methanol or ethanol: Wet PVDF membrane with methanol or ethanol and equilibrate for 10-15 minutes in Thermo Scientific 1-Step Transfer Buffer before transfer.
- Air bubbles trapped between gel and membrane: When assembling transfer stack, use a roller or pipette to remove any air bubbles between the gel and the membrane.
- Filter paper used in the fast transfer exceeded 1.8 mm: Use filter paper <1.8 mm thick.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This is likely due to salt deposited on moving parts inside the cassette. Rinse the cassette top and bottom under warm water while removing any sticky salt residue with a gloved hand. Briefly rinse with deionized water and place in a rack to dry.

For more thorough cleaning, immerse the unassembled cassette in warm water, use a gloved hand or clean sponge to remove any sticky salt residue. Rinse with deionized water and place perpendicular in a rack to dry.

Note: Failure to keep cassette top (cathode) and bottom (anode) clean will result in the sticking of moving parts and lead to poor transfer efficiency.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

When the Thermo Scientific Power Blotter is used in conjunction with Towbin Transfer Buffer, one to four mini-sized gels or one to two midi-sized gels can be simultaneously transferred in 45-60 minutes. Please note that gels simultaneously transferred must have the same formulation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

When the Thermo Scientific Power Blotter is used in conjunction with the Thermo Scientific 1-Step Transfer Buffer, one to four mini-sized gels or one to two midi-sized gels can be simultaneously transferred in 5-10 minutes. Please note that gels simultaneously transferred must have the same formulation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Thermo Scientific Power Blotter (Cat. No. PB0012) consists of the Thermo Scientific Power Station (Cat. No. PB0010) with activated Blotting Software and the Thermo Scientific Power Blot Cassette (Cat. No. PB0002). It is designed for rapid semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes. Traditional western blotting techniques require a transfer of one hour to overnight to achieve good transfer efficiency. When used in conjunction with Thermo Scientific 1-Step Transfer Buffer, the Thermo Scientific Power Blotter is designed to provide transfer efficiency in 5-10 min that is equivalent to, or better than, traditional blotting techniques without the need for gel pre-equilibration. This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buffer and increasing the current [amps (A)/cm2] flowing through the transfer stack. The system has been verified to work with commonly used pre-cast and homemade SDS-PAGE gels. The Thermo Scientific Power Blotter can also be used for standard semi-dry transfer protocols with Towbin buffer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.