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查看更多产品信息 PowerEase™ 300W Power Supply (230 VAC) (Australia) - FAQs (PS0302)
42 个常见问题解答
在NativePAGE电泳期间，电流下降至低于1 mA很常见。PowerEase 300W电源会将此记录为“空载”错误并自动关闭，导致电泳停止。由于PowerEase 300W电源无法禁用/关闭“负载检测”功能，所以我们不推荐将其用于Native-PAGE电泳。
PowerEase 300W电源配有一个备用保险丝。请参见使用手册第19页对更换保险丝的详细说明。
以下是出现该错误的可能原因和解决方案：
- 恒定电压模式改变为恒定电流模式
- 电流极限设置过低，对电流上限产生冲击，从而使恒定电压模式改变为恒定电流模式。应升高电流极限至3 A。
- 恒定电流模式改变为恒定电压模式
- 电压设置过低，对电压上限产生冲击，从而使恒定电流模式改变为恒定电压模式。应升高电压极限值250V。
以下是发生这种情况的可能原因：
- 在电泳过程中增加或移除电泳系统
- 连接系统中出现缓冲液泄漏
- 过度升温
- 过多的缓冲液蒸发
- 连接系统中出现连接松动
- 电流设置过低
以下是可能原因和解决方案：
- 负载超过3 A: 电源的最大输出量为3 A，所以应确保不超过3 A。
- 电源保险丝熔断: 按照使用手册第19页的说明更换保险丝。
- 输入电压错误: 检查电源接入口附近的输入电压开关。
这可能是因为电源过热。请执行以下操作：
- 关闭电源。检查电源风扇周围的气流是否充足。降温后，通过接通电源开关重启电源。
- 如果您无法重启仪器，请关闭电源并从电源插座上拔掉电源线，然后发送邮件至techsupport@lifetech.com来联系技术服务。
这最可能是因为在运行期间检测到接地泄漏。应检查电泳系统是否存在不正确的接地情况，然后关闭和打开电源开关以重启电源。
这表示电路中断。请进行以下检查：
- 确认使用了正确的电泳缓冲液。
- 确认所有电线正确连接。
- 关闭电源并重新启动；重新开启应用。
- 如果您无法重启仪器，请关闭电源并从电源插座上拔掉电源线，然后发送邮件至techsupport@lifetech.com来联系技术服务。
以下是可能原因和解决方案：
- 电泳槽导线未连接到电源或电泳装置，或电泳槽出现短路: 检查电源和电泳槽的连接处，确保连接完好无损；检查电泳装置中的电线。重新连接好电线以接通电路。按开始/暂停按钮，重新开始电泳。
- 由预制胶上的胶带残留、错误的缓冲液浓度或电泳槽中错误的缓冲液体积导致的高电阻: 应确保移除预制胶上的胶带、正确配制缓冲液并在电泳装置中加入推荐的缓冲液体积。
- 电流低于可接受额定值（10 mA）: 电源具有内置的“空载检测”功能，可随时检测电流是否低于可接受额定值10 mA。
以下是可能原因和解决方案：
- 交流电电源线未连接: 检查交流电电源线两端的连接情况。使用正确的电源线。
- 保险丝熔断: 更换保险丝。
关于更换PowerEase 300W电源保险丝的详细说明，请参考使用手册(https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf)第19页。
PowerEase 300W电源不需要定期维护，只需偶尔用干布擦拭仪器即可。
PowerEase 300W电源可在限制电压、限制电流或限制功率条件下运行。但是，对于大部分应用，我们推荐在限制电压下操作，因为这具有以下优势：
•对于大部分电泳方法，电阻在整个电泳过程中不断增加。在限制电压下，电流和功率在整个电泳过程中不断降低，从而能够随时间不断提高安全性。
•即使同时进行电泳的凝胶数量和厚度不同，也可使用相同的电压设置。
注意：
根据欧姆定律（V=IR），在限制电流设置下，电压会随电阻的增加而升高。如果在不限制电压的情况下出现局部故障，如连接不良，则非常高的局部电阻可能导致电压升高并达到电源的最大容量。这可能引起局部过热，并损害电泳槽或产生不安全因素。在恒定电流下运行时，我们建议设置电源的电压极限等于或稍高于最大预期电压。
功率与电压和电流呈函数关系，即P=IV。对于标准凝胶电泳，在恒定功率下，电流会降低并引起电压升高。当为了除去上样孔中的过硫酸铵而进行测序凝胶电泳并加热凝胶以获得DNA分离的最佳温度时，可使用功率限制函数。
当使用PowerEase 300W电源进行1-2块蛋白质凝胶的电泳/转印时，我们推荐使用恒定电压模式以获得最佳性能。
为获得最佳结果，我们不推荐在最大电力负载极限下使用PowerEase 300W电源。缓冲液条件的变化可导致电压、电流或功率输出容量超出电源最大限制，对性能造成不良影响。
PowerEase 300W电源的产品规格见使用手册(https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf)第18页。
PowerEase 300W电源包含以下组件：
•PowerEase 300W电源
•备用保险丝
•电源线
注意：请使用您所在国家/地区允许使用的电源线。北美地区的客户应使用B型电源线；日本的客户应使用JIS 8303电源线；中国的客户应使用GB 15934-2008电源线。澳大利亚/新西兰的客户应使用I型电源线。
PowerEase 300W电源可容纳8块小型凝胶或16块中型凝胶的电泳和转印。
PowerEase 300W电源是一种全编程、微处理器控制的电源，专为高通量凝胶电泳设计。该电源界面直观，并具有以下特点：
•恒定的电压、电流或功率设置
•内置计时器，实现可离开式凝胶电泳
•多达10个自定义程序，每个程序10个步骤
•四套输出插孔，可兼容大多数电泳设备
Yes, the PowerEase 300W Power Supply (230 VAC) (Cat. No. PS0301) as well as the PowerEase 90W Power Supply (Cat. No. PS0090) are both compatible with the Owl chambers; confirmed for the Owl EasyCast B2 Mini Gel Electrophoresis System (Cat. No. B2) and the Owl A2 Large Gel System, 2.3L, 37.5 x 32.5 x 10.5cm (L x W x H) (Cat. No. A2). Also, vice versa, Owl 300XL power supply + adapters can be used with the Mini Gel Tank (Cat. No. A25977), XCell SureLock Mini-Cell (Cat. No. EI0001) and XCell4 SureLock Midi-Cell (Cat. No. WR0100).
Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.
This is not really recommended, but it is always possible to increase run time by lowering the voltage of the run. In general, the relationships are linear - i.e., decreasing voltage by half will double the run time.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
If you are running the gel at constant voltage, you do not need to increase the voltage regardless of the thickness of the gel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Using constant voltage allows the current and power to decrease during the run, providing a safety margin in case of a break in the system. Having lower power is a safety benefit and will also decrease the chances of experiencing an overheating of the gel. Further, the constant voltage setting does not need adjustment to account for differences in number or thickness of gels being run.
Find additional tips, troubleshooting help, and resources within ourProtein Gel 1D Electrophoresis Support Center.
During NativePAGE electrophoresis, it is common for the current to drop below 1 mA. The PowerEase 300W Power Supply will register this as a “No Load” error and automatically shut off, resulting in the stopping of the gel run. Since there is no way to disable/turn off the “Load Check” feature in the PowerEase 300W Power Supply, we do not recommend using it for Native-PAGE electrophoresis. Alternatively, the ZOOM Dual Power Supply is capable of disabling the “Load Check” feature and is our recommended power supply for NativePAGE applications.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
One extra fuse is supplied with the PowerEase 300W Power Supply. For detailed instructions on replacing the fuse, please refer to Page 19 of the manual.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are the scenarios for this error with likely causes and solutions:
- Voltage changes to current
- Current limit was set too low due to which the ceiling for current was hit and constant mode changed from voltage to current. Increase current limit to 3 A.
- Current changes to voltage
- Voltage was set to low due to which the ceiling for voltage was hit and constant mode changed from current to voltage. Increase voltage limit to 250 V.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are likely reasons why this might happen:
- Electrophoresis systems were added or removed during a run
- Buffer leaking in a connected system
- Excessive temperature increase
- Excessive buffer evaporation
- Loose connection in a connected system
- Current set too low
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Load exceeds 3 A. The output maximum on the power supply is 3 A, so make sure that it is not exceeded.
- Blown fuse in the power supply. Replace fuse following instructions on Page 19 of the manual.
- Incorrect input voltage. Check input voltage switch near power inlet.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This is due to overheating of the power supply. Please do the following:
- Turn off power supply. Check for sufficient airflow around the power supply fan. After cooling down, restart the power supply by turning the Power switch to the on position.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service by sending an e-mail to techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This is most likely due to a ground leak detected during the run. Check the electrophoresis system for improper grounding and restart the power supply by turning the Power switch off and on.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This indicates that the circuit has been interrupted. Please check the following:
- Verify that the running buffer is correct.
- Verify that all cables are attached correctly.
- Turn the Power switch off and on again; restart application.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service by sending an e-mail to techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Electrophoresis leads are not connected to the power supply or to the electrophoresis unit(s), or there is a broken circuit in the electrophoresis cell. Check the connections to the power supply and on your electrophoresis cell to make sure the connection is intact; check condition of wires in electrophoresis unit. Close the circuit by reconnecting the cables. Press Start/Pause to restart the run.
- High resistance due to tape left on a pre-cast gel, incorrect buffer concentration, or incorrect buffer volumes in the electrophoresis cell. Correct the condition by making sure the tape is removed from the pre-cast gel, buffers are prepared correctly, and the recommended volume of buffer is added to the electrophoresis unit.
- Current has dropped below acceptable rating (10 mA). The power supply has a built-in “No Load Detection” feature that can detect when the current drops below the acceptable rating of 10 mA.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- AC power cord is not connected. Check AC power cord connections at both ends. Use the correct cord.
- The fuse has blown. Replace the fuse.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
For detailed instructions on replacing the fuse on the PowerEase 300W Power Supply, please refer to Page 19 of the manual (https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply requires no periodic maintenance with the exception of an occasional dry wipe-down of the instrument.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply is capable of operating at limiting voltage, limiting current, or limiting power. However, we recommend operating it at limiting voltage for most applications as it provides the following advantages:
- For most electrophoresis methods, resistance increases throughout the run. Under limiting voltage, current and power decrease throughout the run, providing an improving margin of safety over time.
- The same voltage setting can be used regardless of the number or thickness of gels being electrophoresed.
Note: With the current limiting setting, the voltage will increase as resistance increases to satisfy Ohm's law (V=IR). If no voltage limit is set and a local fault condition occurs, such as a poor connection, very high local resistance may cause the voltage to increase to the maximum capacity of the power supply. This may lead to local overheating and damage to the electrophoresis cell or create unsafe conditions. When operating under constant current conditions, we recommend setting a voltage limit on the power supply at or slightly above the maximum expected voltage. Power is a function of voltage and current P=IV. For a typical gel run, the current will decrease causing the voltage to increase when run at constant power. The power limiting function may be used when running sequencing gels to remove the ammonium persulfate from the wells and to heat the gel to an optimal temperature for DNA separations.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
When using the PowerEase 300W Power Supply with 1-2 proteins gels or blots, we recommend using the Constant Voltage mode for optimal performance.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
For best results, we do not recommend using the PowerEase 300W Power Supply at its maximum electrical load limits. Variations in buffer conditions can result in exceeding the power supply's maximum voltage, current, or power output capacity and produce undesirable variations in performance.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The product specifications for the PowerEase 300W Power Supply are listed on Page 18 of the manual (https://tools.thermofisher.com/content/sfs/manuals/PowerEase300W_UG_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply includes the following components:
- PowerEase 300W Power Supply
- Extra fuse
- Power cord
Note: Please use the power cord approved for your country/region. North America customers should use the Type B power cord; Japanese customers should use the JIS 8303 power cord; Chinese customers should use the GB 15934-2008 power cord; Australia/New Zealand customers should use the Type I power cord.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply accommodates the running and transferring of 8 Mini gels, or 16 Midi gels.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 300W Power Supply is a fully programmable, microprocessor-controlled power supply designed for high-throughput gel electrophoresis. It has a straight-forward, intuitive interphase and includes the following features:
- Constant voltage, current, or power settings
- Built-in timer for walk-away gel electrophoresis
- Up to 10 custom programs with 10 steps each
- Four sets of output jacks that are compatible with most electrophoresis devices
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.