A dual estrogen receptor TR-FRET assay for simultaneous measurement of steroid site binding and coactivator recruitment.
AuthorsHilal T, Puetter V, Otto C, Parczyk K, Bader B,
JournalJ Biomol Screen
PubMed ID20150592
The human estrogen receptors (hER) are members of the nuclear hormone receptor (NHR) superfamily and represent important drug targets for the pharmaceutical industry. Initially, ligand binding assays were used to identify novel ligands using receptors purified from native tissues. With the advent of molecular cloning techniques, cell-based transactivation assays have ... More