BacMam Enhancer Solution (1000X) - FAQs

查看更多产品信息 BacMam Enhancer Solution (1000X) - FAQs (PV5835)

2 个常见问题解答

我使用你们的CellLight BacMam 试剂对我的细胞器进行荧光蛋白标记，但是标记效果不佳。我该如何加以改善？

首先，确定你们的细胞是哺乳动物且非血源性细胞。如果细胞转导效率不佳（例如只有少数细胞被标记或是标记荧光很暗），先试着增加每个细胞的微粒数。接下来试着使用BacMam Enhancer Solution。但是，如果你们的细胞显示标记而标记定位似乎太过普遍或是无处不在，那么你们应该降低每个细胞的微粒数。该手册给出了计算每个细胞的微粒数的指南和公式。

I am using your CellLight BacMam reagents for fluorescent protein-labeling of my cell organelles, but they aren't labeling well. How can I improve it?

First, make sure that your cells are mammalian and non-blood derived. If the cells have low transduction efficiency (i.e., only a few cells are labeling or are labeling only dimly), first try increasing the particle number per cell. The next thing to try is to use the BacMam Enhancer Solution. If, however, your cells show labeling, but the localization seems to be too generalized or ubiquitous within the cell, then you should reduce the particle number per cell. The manual gives a guideline and formula for calculating particle number per cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.