Search
PolarScreen™ PPARγ 竞争剂检测试剂盒，绿色
The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal了解更多信息
| 货号 | 数量 |
|---|---|
| PV6136 | 800 x 20 μL |
货号 PV6136
价格（CNY)
26,796.00
Each
数量:
800 x 20 μL
价格（CNY)
26,796.00
Each
The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.
仅供科研使用。不可用于诊断程序。
规格
检测条目生物化学竞争性结合
颜色绿色
检测方法荧光
激发/发射485/535
适用于（应用）竞争性结合检测，荧光偏振
适用于（设备）微孔板读数仪
基因 ID (Entrez)5468
配体PPAR
包装384 孔板
产品类型检测试剂盒
数量800 x 20 μL
读值终点法
运输条件干冰
目标条目PPARG、PPAR γ、NR1C3
产品线PolarScreen™
Unit SizeEach
内容与储存
PV3356 — Fluormone™ PPARγ Green (-20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green 筛选缓冲液（室温）
P2325 — 1M DTT（-80°C 或 -20°C）
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green 筛选缓冲液（室温）
P2325 — 1M DTT（-80°C 或 -20°C）