Invitrogen

Human ABL1 [H396P], GST Tag, His Tag Recombinant Protein

Name: Human ABL1 [H396P], GST Tag, His Tag Recombinant Protein
Brand: Invitrogen
SKU: PV6150

说明书

技术支持

说明书

技术支持

产品信息

PV6150

产品规格

种属

Human

Expression System

Insect cells

氨基酸序列

118-535

分子量

76.1 kDa

分类

Recombinant

类型

Protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

See Label

纯化类型

purified

保存液

50mM HEPES, pH 7.5, with 20% glycerol, 5mM DTT, 15mM glutathione, 100mM NaCl

内含物

no preservative

保存条件

-80°C, Avoid Freeze/Thaw Cycles

运输条件

Dry ice

产品详细信息

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Human ABL1, internal fragment, amino acids P118-S535 (as in GenBank entry NM_005157.3), H396P mutant, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant

靶标信息

c-Abl (Abelson murine leukemia viral oncogene homolog 1, ABL1) is a 140 kDa proto-oncogene member of the Src family of non-receptor tyrosine kinases. c-Abl has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns c-Abl into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and c-Abl genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for c-Abl. In chronic myelogenous leukemia and a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. The c-Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The c-Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. c-Abl localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. c-ABL potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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