Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots.
AuthorsGiepmans BN, Deerinck TJ, Smarr BL, Jones YZ, Ellisman MH
JournalNat Methods
PubMed ID16179920
'The importance of locating proteins in their context within cells has been heightened recently by the accomplishments in molecular structure and systems biology. Although light microscopy (LM) has been extensively used for mapping protein localization, many studies require the additional resolution of the electron microscope. Here we report the application ... More
Near-complete suppression of quantum dot blinking in ambient conditions.
AuthorsHohng S, Ha T
JournalJ Am Chem Soc
PubMed ID14759174
Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection.
AuthorsNess JM, Akhtar RS, Latham CB, Roth KA
JournalJ Histochem Cytochem
PubMed ID12871979
Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple ... More
A photoluminescence-based field method for detection of traces of explosives.
AuthorsMenzel ER, Menzel LW, Schwierking JR
JournalScientificWorldJournal
PubMed ID15349512
We report a photoluminescence-based field method for detecting traces of explosives. In its standard version, the method utilizes a commercially available color spot test kit for treating explosive traces on filter paper after swabbing. The colored products are fluorescent under illumination with a laser that operates on three C-size flashlight ... More